Sequential cancer mutations in cultured human intestinal stem cells

  title={Sequential cancer mutations in cultured human intestinal stem cells},
  author={Jarno Drost and Richard H. van Jaarsveld and Bas Ponsioen and Cheryl Doreen Zimberlin and Ruben van Boxtel and Arjan Buijs and Norman Sachs and Ren{\'e} M. Overmeer and George Johan Arnold Offerhaus and Harry Begthel and Jeroen Korving and Marc van de Wetering and Gerald Schwank and Meike E. W. Logtenberg and Edwin Cuppen and Hugo J. G. Snippert and Jan Paul Medema and Geert J. P. L. Kops and Hans Clevers},
Crypt stem cells represent the cells of origin for intestinal neoplasia. Both mouse and human intestinal stem cells can be cultured in medium containing the stem-cell-niche factors WNT, R-spondin, epidermal growth factor (EGF) and noggin over long time periods as epithelial organoids that remain genetically and phenotypically stable. Here we utilize CRISPR/Cas9 technology for targeted gene modification of four of the most commonly mutated colorectal cancer genes (APC, P53 (also known as TP53… CONTINUE READING
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