Sequential biochemical and morphological events during assembly of the fertilization membrane of the sea urchin.

@article{Veron1977SequentialBA,
  title={Sequential biochemical and morphological events during assembly of the fertilization membrane of the sea urchin.},
  author={M. Veron and C A Foerder and Edward M. Eddy and S C Shapiro},
  journal={Cell},
  year={1977},
  volume={10 2},
  pages={321-8}
}
The fertilization membrane of Strongylocentrotus purpuratus undergoes changes in morphology, solubility, and permeability during the process of hardening. As the fertilization membrane elevates from the egg surface, it retains casts of the tips of the microvillous processes of the plasma membrane. The dome-shaped microvillar casts become angular at the same time that the fertilization membrane becomes resistant to solubilization in mercaptan solutions. 2-4 min after this morphological and… CONTINUE READING
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2 - 4 min after this morphological and chemical transition , the fertilization membrane becomes impermeable to the lectin conconavalin A , as monitored by binding of 125I- or fluorescein - labeled concanavalin A. Glycine ethyl ester inhibits the changes in morphology , solubility , and permeability , whereas sodium sulfite inhibits only the permeability block and resistance to solubilization by mercaptans .
2 - 4 min after this morphological and chemical transition , the fertilization membrane becomes impermeable to the lectin conconavalin A , as monitored by binding of 125I- or fluorescein - labeled concanavalin A. Glycine ethyl ester inhibits the changes in morphology , solubility , and permeability , whereas sodium sulfite inhibits only the permeability block and resistance to solubilization by mercaptans .
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