• Biology, Medicine
  • Published in
    The Journal of biological…
    1983

Sequential assembly of very low density lipoprotein apolipoproteins, triacylglycerol, and phosphoglycerides by the intact liver cell.

@article{Janero1983SequentialAO,
  title={Sequential assembly of very low density lipoprotein apolipoproteins, triacylglycerol, and phosphoglycerides by the intact liver cell.},
  author={David R Janero and Melissa D. Lane},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 23},
  pages={
          14496-504
        }
}
Primary cultures of estrogen-induced chick parenchymal liver cells have been used to study the assembly of the apolipoprotein and glycerolipid constituents of hepatic very low density lipoprotein (VLDL). Liver cell monolayers in serum-free medium were pulse-labeled for 2.5 min with either [3H]leucine or [3H]glycerol and were then chased for 4 h. The kinetics of secretion of VLDL 3H-apolipoproteins (labeled from [3H]leucine) and [3H]triacylglycerol and 3H-phosphoglycerides (labeled from [3H… CONTINUE READING

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