Sequential and simultaneous statistical optimization by dynamic design of experiment for peptide overexpression in recombinant Escherichia coli.

@article{Lee2006SequentialAS,
  title={Sequential and simultaneous statistical optimization by dynamic design of experiment for peptide overexpression in recombinant Escherichia coli.},
  author={Kwang-Min Lee and Chang-Hoon Rhee and Choong-Kyung Kang and Jung-Hoe Kim},
  journal={Applied biochemistry and biotechnology},
  year={2006},
  volume={135 1},
  pages={59-80}
}
The production of recombinant anti-HIV peptide, T-20, in Escherichia coli was optimized by statistical experimental designs (successive designs with multifactors) such as 2(4-1) fractional factorial, 2(3) full factorial, and 2(2) rotational central composite design in order. The effects of media compositions (glucose, NPK sources, MgSO4, and trace elements), induction level, induction timing (optical density at induction process), and induction duration (culture time after induction) on T-20… CONTINUE READING

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