Sequential and multistep substrate interrogation provides the scaffold for specificity in human flap endonuclease 1.

@article{Sobhy2013SequentialAM,
  title={Sequential and multistep substrate interrogation provides the scaffold for specificity in human flap endonuclease 1.},
  author={Mohamed A. Sobhy and Luay I Joudeh and Xiaojuan Huang and Masateru Takahashi and Samir M Hamdan},
  journal={Cell reports},
  year={2013},
  volume={3 6},
  pages={1785-94}
}
Human flap endonuclease 1 (FEN1), one of the structure-specific 5' nucleases, is integral in replication, repair, and recombination of cellular DNA. The 5' nucleases share significant unifying features yet cleave diverse substrates at similar positions relative to 5' end junctions. Using single-molecule Förster resonance energy transfer, we find a multistep mechanism that verifies all substrate features before inducing the intermediary-DNA bending step that is believed to unify 5' nuclease… CONTINUE READING
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The wonders of flap endonucleases: structure, function, mechanism and regulation.

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