Sequential Passages of Human Rotavirus in MA‐104 Cells
@article{Urasawa1981SequentialPO, title={Sequential Passages of Human Rotavirus in MA‐104 Cells}, author={Tomoko Urasawa and Shozo Urasawa and Koki Taniguchi}, journal={Microbiology and Immunology}, year={1981}, volume={25} }
Starting with a small amount of diarrheal feces containing human rotavirus (HRV), we succeeded in propagation of the virus using the roller culture technique with MA‐104 cells. Furthermore, we made a successful adaptation of HRV to a stationary culture and developed a plaque assay for the cell culture‐adapted viruses. The 3 culture‐adapted virus isolates, KU, YO, and 44 produced plaques (about 0.5–1.0 mm in diameter) under the overlay medium consisting of 0.6% purified agar, 3 μg of acetyl…
173 Citations
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ヒトレオウイルス様因子 (HRLA) あるいはウシレオウイルス様因子 (NCDV) を抗原に用いて測定した下痢症患者ならびに非患者の年齢別血清抗体価の比較検討の結果, 次のような成績を得た.1. 患者血清ではHRLA-CF抗体とNCDV-CF, -PT (counter-immunoelectro-osmophoresis による沈降反応), -NT (中和)…