Sequential Passages of Human Rotavirus in MA‐104 Cells

  title={Sequential Passages of Human Rotavirus in MA‐104 Cells},
  author={Tomoko Urasawa and Shozo Urasawa and Koki Taniguchi},
  journal={Microbiology and Immunology},
Starting with a small amount of diarrheal feces containing human rotavirus (HRV), we succeeded in propagation of the virus using the roller culture technique with MA‐104 cells. Furthermore, we made a successful adaptation of HRV to a stationary culture and developed a plaque assay for the cell culture‐adapted viruses. The 3 culture‐adapted virus isolates, KU, YO, and 44 produced plaques (about 0.5–1.0 mm in diameter) under the overlay medium consisting of 0.6% purified agar, 3 μg of acetyl… 
Replication of human rotavirus in cell culture
Nine strains of human rotavirus were adapted to growth in CV‐1 and/or MA‐104 cells following pretreatment of virus with trypsin, incorporation of trypsin into culture medium, and use of roller
Isolation of human rotaviruses in primary cultures of monkey kidney cells
The cultivation of human rotaviruses in primary cell cultures might aid in developing a liver rotavirus vaccine.
Isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (MA104) cell line.
Turkey rotaviruses from the intestinal contents of poults were isolated and serially propagated in MA104 cell monolayers by a simple procedure and possessed the morphologic, antigenic, and genomic attributes characteristic of turkey group A rotavirus.
Serological characterisation of human rotaviruses propagated in cell cultures
Fourteen strains of human rotaviruses were propagated in MA 104 cell culture using serum-free medium containing 10 µg/ml trypsin and appeared to be related to a South American rotavirus strain reported in 1978 as a probable fourth serotype.
Efficiency of human rotavirus propagation in cell culture
Primary cells supported virus growth directly from fecal specimens much more efficiently than did continuous lines of African green or rhesus monkey kidney cells, and passage in these cells both increased virus infectivity and adapted the viruses for growth in continuous cell lines.
Isolation of human rotavirus subgroups 1 and 2 in cell culture
One strain of human rotavirus subgroup 1 (KUN) and one strain of subgroup 2 (MO) were isolated with the MA104 cell line, a fetal rhesus monkey kidney cell line. Their subgroup specificities and RNA
Cell culture propagation of rotaviruses
Optimal conditions for the growth of two group A rotaviruses (OSU & NCDV) in MA-104 cells are described in this report.


Human rotavirus type 2: cultivation in vitro.
A strain of type 2 human rotavirus (Wa) was grown to relatively high titer through 14 passages in primary cultures of African green monkey kidney (AGMK) cells. This passage series was initiated with
Cell culture propagation of neonatal calf diarrhea (scours) virus.
EXPERIMENTAL PRODUCTION of neonatal calf diarrhea with a virus in calves contaminated with and free of E. coli has been reported (1) . Measured by electron microscopy, the virus had a diameter of 65
Simian rotavirus SA-11 plaque formation in the presence of trypsin
Incorporation of 5 micrograms of trpsin per ml of the overlay (Eagle minimal essential medium-0.7% Ionagar no. 2) was found to be necessary for plaque formation by simian rotavirus SA-11 and should further enhance its usefulness in this regard.
Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera
It was demonstrated that some human sera contained neutralizing antibody to this agent, and titration of neutralizing antibodies to neonatal calf diarrhea virus was made feasible.
Virus-like particle, 35 to 40 nm, associated with an institutional outbreak of acute gastroenteritis in adults
The possibility that this agent, tentatively designated as the Otofuke agent, might be a new candidate virus for gastroenteritis might be suggested.
Epidemiology of human rotavirus Types 1 and 2 as studied by enzyme-linked immunosorbent assay.
An enzyme-linked immunosorbent assay to differentiate serotype-specific rotavirus antigen and antibody suggests that, to be completely effective, a vaccine must provide resistance to both serotypes.
A long-term survey of rotavirus infection in Japanese children with acute gastroenteritis.
The results indicate that human rotavirus can be regarded as a major etiologic agent of acute gastroenteritis in infants and young children, of which wintertime epidemics are common in Japan.
[Utility of Nebraska calf diarrhea virus (NCDV) antigen in detection of antibody against human Reovirus-like agent (author's transl)].
ヒトレオウイルス様因子 (HRLA) あるいはウシレオウイルス様因子 (NCDV) を抗原に用いて測定した下痢症患者ならびに非患者の年齢別血清抗体価の比較検討の結果, 次のような成績を得た.1. 患者血清ではHRLA-CF抗体とNCDV-CF, -PT (counter-immunoelectro-osmophoresis による沈降反応), -NT (中和)