Sequential GC/MS analysis of sialic acids, monosaccharides, and amino acids of glycoproteins on a single sample as heptafluorobutyrate derivatives.

@article{Pons2003SequentialGA,
  title={Sequential GC/MS analysis of sialic acids, monosaccharides, and amino acids of glycoproteins on a single sample as heptafluorobutyrate derivatives.},
  author={Alexandre Pons and Colette Richet and Catherine Robbe and Annkatrin Herrmann and Philippe Timmerman and G. Huet and Yves Leroy and Ingemar Carlstedt and C. A. F. M. Gr{\"u}tters J. A. P. J. Breuker H. J. van de Capon and J. P. Zanetta},
  journal={Biochemistry},
  year={2003},
  volume={42 27},
  pages={8342-53}
}
A GC/MS procedure was developed for the analysis of all major constituents of glycoproteins. The rationale for this approach is that by using GC/MS analysis of the constituents as heptafluorobutyrate derivatives, it was possible to quantitatively determine the sialic acid, monosaccharide, fatty acids (when present), and the amino acid composition with the sample remaining in the same reaction vessel during the entire procedure. A mild acid hydrolysis was used to liberate sialic acids and was… CONTINUE READING