Sequential Analysis of Trans-SNARE Formation in Intracellular Membrane Fusion

  title={Sequential Analysis of Trans-SNARE Formation in Intracellular Membrane Fusion},
  author={Kannan Alpadi and Aditya Kulkarni and Veronique Comte and Monique Reinhardt and Andrea Schmidt and Sarita Namjoshi and Andreas Mayer and Christopher G. Peters},
  booktitle={PLoS biology},
SNARE complexes are required for membrane fusion in the endomembrane system. They contain coiled-coil bundles of four helices, three (Q(a), Q(b), and Q(c)) from target (t)-SNAREs and one (R) from the vesicular (v)-SNARE. NSF/Sec18 disrupts these cis-SNARE complexes, allowing reassembly of their subunits into trans-SNARE complexes and subsequent fusion. Studying these reactions in native yeast vacuoles, we found that NSF/Sec18 activates the vacuolar cis-SNARE complex by selectively displacing… CONTINUE READING
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