Sequencing viral genomes from a single isolated plaque

  title={Sequencing viral genomes from a single isolated plaque},
  author={Jessica DePew and Bin Zhou and Jamison M. McCorrison and David E. Wentworth and Janaki Purushe and Galina A. Koroleva and Derrick E. Fouts},
  booktitle={Virology Journal},
Whole genome sequencing of viruses and bacteriophages is often hindered because of the need for large quantities of genomic material. A method is described that combines single plaque sequencing with an optimization of Sequence Independent Single Primer Amplification (SISPA). This method can be used for de novo whole genome next-generation sequencing of any cultivable virus without the need for large-scale production of viral stocks or viral purification using centrifugal techniques. A single… CONTINUE READING
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View 9 Excerpts
Highly Influenced

Cutadapt removes adapter sequences from high-throughput sequencing reads

M Martin
EMBnetjournal • 2011
View 2 Excerpts
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High-quality draft assemblies of mammalian genomes from massively parallel sequence data.

Proceedings of the National Academy of Sciences of the United States of America • 2011
View 2 Excerpts
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PEG precipitation for selective removal of small DNA fragments

JL Hartley, H Bowen
Focus • 1996
View 3 Excerpts
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