Sequencing viral genomes from a single isolated plaque

@inproceedings{DePew2013SequencingVG,
  title={Sequencing viral genomes from a single isolated plaque},
  author={Jessica DePew and Bin Zhou and Jamison M. McCorrison and David E. Wentworth and Janaki Purushe and Galina A. Koroleva and Derrick E. Fouts},
  booktitle={Virology Journal},
  year={2013}
}
Whole genome sequencing of viruses and bacteriophages is often hindered because of the need for large quantities of genomic material. A method is described that combines single plaque sequencing with an optimization of Sequence Independent Single Primer Amplification (SISPA). This method can be used for de novo whole genome next-generation sequencing of any cultivable virus without the need for large-scale production of viral stocks or viral purification using centrifugal techniques. A single… CONTINUE READING
Related Discussions
This paper has been referenced on Twitter 7 times. VIEW TWEETS

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.

References

Publications referenced by this paper.
Showing 1-10 of 26 references

Viral genome sequencing by random priming methods

BMC Genomics • 2007
View 9 Excerpts
Highly Influenced

Cutadapt removes adapter sequences from high-throughput sequencing reads

M Martin
EMBnetjournal • 2011
View 2 Excerpts
Highly Influenced

High-quality draft assemblies of mammalian genomes from massively parallel sequence data.

Proceedings of the National Academy of Sciences of the United States of America • 2011
View 2 Excerpts
Highly Influenced

PEG precipitation for selective removal of small DNA fragments

JL Hartley, H Bowen
Focus • 1996
View 3 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…