Sequencing of the tuf1 gene and the phosphorylation pattern of EF-Tu1 during development and differentiation in Streptomyces collinus producing kirromycin.

@article{Mikulk1995SequencingOT,
  title={Sequencing of the tuf1 gene and the phosphorylation pattern of EF-Tu1 during development and differentiation in Streptomyces collinus producing kirromycin.},
  author={K. Mikul{\'i}k and E. Zhulanova},
  journal={Biochemical and biophysical research communications},
  year={1995},
  volume={213 2},
  pages={
          454-61
        }
}
  • K. Mikulík, E. Zhulanova
  • Published 1995
  • Biology, Medicine
  • Biochemical and biophysical research communications
  • We have cloned and sequenced the tuf1 gene from a kirromycin-producing strain of Streptomyces collinus. The gene encodes a polypeptide of 396 amino acid residues with a molecular weight of 43,849. The protein shows 97% identity with EF-Tu1 of S. coelicolor and is sensitive to kirromycin. EF-Tu-dependent translation of poly(U) was reduced to 50% in the presence of 0.25 microM kirromycin. Using high resolution two-dimensional electrophoresis and specific immunodetection with monoclonal antibodies… CONTINUE READING
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