Sequencing of rat liver cytosolic proteins by matrix-assisted laser desorption ionization-quadrupole time-of-flight mass spectrometry following electrophoretic separation and extraction.

@article{She2002SequencingOR,
  title={Sequencing of rat liver cytosolic proteins by matrix-assisted laser desorption ionization-quadrupole time-of-flight mass spectrometry following electrophoretic separation and extraction.},
  author={Y M She and G. Wang and Alexander V Loboda and Werner E Ens and Kenneth G Standing and Frank J. Burczynski},
  journal={Analytical biochemistry},
  year={2002},
  volume={310 2},
  pages={137-47}
}
A new technique is described that enables the direct determination of the complete or partial amino acid sequence of cytosolic proteins separated by gel electrophoresis and allows for the further observation of disease- or drug-induced posttranslational modifications. The procedure uses a two-phase extraction strategy (ethyl acetate/ammonium bicarbonate) for the efficient separation of proteins/peptides from an electrophoretic matrix and subsequent sequence analysis by matrix-assisted laser… CONTINUE READING

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