Sequencing genomes from single cells by polymerase cloning

  title={Sequencing genomes from single cells by polymerase cloning},
  author={K. Zhang and A. Martiny and N. Reppas and K. Barry and J. Malek and S. Chisholm and G. Church},
  journal={Nature Biotechnology},
  • K. Zhang, A. Martiny, +4 authors G. Church
  • Published 2006
  • Biology, Medicine
  • Nature Biotechnology
  • Genome sequencing currently requires DNA from pools of numerous nearly identical cells (clones), leaving the genome sequences of many difficult-to-culture microorganisms unattainable. We report a sequencing strategy that eliminates culturing of microorganisms by using real-time isothermal amplification to form polymerase clones (plones) from the DNA of single cells. Two Escherichia coli plones, analyzed by Affymetrix chip hybridization, demonstrate that plonal amplification is specific and the… CONTINUE READING
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