Sequencing genomes from single cells by polymerase cloning

@article{Zhang2006SequencingGF,
  title={Sequencing genomes from single cells by polymerase cloning},
  author={Kun Qiu Zhang and Adam C. Martiny and Nikos B. Reppas and Kerrie W Barry and Joel A. Malek and Sallie W. Chisholm and George M Church},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={680-686}
}
Genome sequencing currently requires DNA from pools of numerous nearly identical cells (clones), leaving the genome sequences of many difficult-to-culture microorganisms unattainable. We report a sequencing strategy that eliminates culturing of microorganisms by using real-time isothermal amplification to form polymerase clones (plones) from the DNA of single cells. Two Escherichia coli plones, analyzed by Affymetrix chip hybridization, demonstrate that plonal amplification is specific and the… CONTINUE READING
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