Sequencing and expression analysis of the sakacin P bacteriocin produced by a Lactobacillus sakei strain isolated from naturally fermented sausages

@article{Urso2005SequencingAE,
  title={Sequencing and expression analysis of the sakacin P bacteriocin produced by a Lactobacillus sakei strain isolated from naturally fermented sausages},
  author={Rosalinda Urso and Kalliopi Rantsiou and Carlo Cantoni and Giuseppe Comi and Luca Cocolin},
  journal={Applied Microbiology and Biotechnology},
  year={2005},
  volume={71},
  pages={480-485}
}
A Lactobacillus sakei strain, designated as I151 and isolated from naturally fermented sausages, was found to produce the sakacin P bacteriocin which is active against Listeria monocytogenes. In this study, we performed the sequencing of the gene cluster involved in the production of the sakacin P, and we followed the expression of the sppA gene, encoding for the bacteriocin, in vitro, using Rogosa–Sharpe medium, and in situ, inoculating the strain in fermented sausages as starter culture. The… CONTINUE READING

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