Sequencing and characterization of oligosaccharides using infrared multiphoton dissociation and boronic acid derivatization in a quadrupole ion trap.

@article{Pikulski2007SequencingAC,
  title={Sequencing and characterization of oligosaccharides using infrared multiphoton dissociation and boronic acid derivatization in a quadrupole ion trap.},
  author={Michael Pikulski and Amanda E Hargrove and Shagufta H Shabbir and Eric V Anslyn and Jennifer S Brodbelt},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2007},
  volume={18 12},
  pages={2094-106}
}
A simplified method for determining the sequence and branching of oligosaccharides using infrared multiphoton dissociation (IRMPD) in a quadrupole ion trap (QIT) is described. An IR-active boronic acid (IRABA) reagent is used to derivatize the oligosaccharides before IRMPD analysis. The IRABA ligand is designed to both enhance the efficiency of the derivatization reaction and to facilitate the photon absorption process. The resulting IRMPD spectra display oligosaccharide fragments that are… CONTINUE READING