Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.

@article{Ast2001SequencesUO,
  title={Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.},
  author={Gil Ast and Thomas Pavelitz and Alan M. Weiner},
  journal={Nucleic acids research},
  year={2001},
  volume={29 8},
  pages={
          1741-9
        }
}
Three different base paired stems form between U2 and U6 snRNA over the course of the mRNA splicing reaction (helices I, II and III). One possible function of U2/U6 helix II is to facilitate subsequent U2/U6 helix I and III interactions, which participate directly in catalysis. Using an in vitro trans-splicing assay, we investigated the function of sequences located just upstream from the branch site (BS). We find that these upstream sequences are essential for stable binding of U2 to the… CONTINUE READING

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