Sequences of versatile broad-host-range vectors of the RK2 family.

@article{Scott2003SequencesOV,
  title={Sequences of versatile broad-host-range vectors of the RK2 family.},
  author={Heather N. Scott and Philip D. Laible and Deborah K. Hanson},
  journal={Plasmid},
  year={2003},
  volume={50 1},
  pages={74-9}
}
Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences… CONTINUE READING

From This Paper

Topics from this paper.
9 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

Similar Papers

Loading similar papers…