Sequences of alamethicins F30 and F50 reconsidered and reconciled

@article{Kirschbaum2003SequencesOA,
  title={Sequences of alamethicins F30 and F50 reconsidered and reconciled},
  author={Jochen Kirschbaum and Corina Krause and Ruth K Winzheimer and Hans Br{\"u}ckner},
  journal={Journal of Peptide Science},
  year={2003},
  volume={9}
}
From the culture broth of the mould Trichoderma viride, strain NRRL 3199, a microheterogeneous mixture of the membrane active 20‐residue peptaibol alamethicin (ALM) could be isolated. ALMs were isolated by XAD‐2 column chromatography and separated by silica gel chromatography and trichloromethane/MeOH gradient elution into an acidic and neutral group of peptides, named ALM F30 and ALM F50, respectively, according to their 100 Rf on TLC. Peptides ALM F50 were separated by semi‐preparative and… 
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Monitoring production and degradation of peptaibiotics in a pilot experiment revealed that the biosynthesis of TB II and TB III peptides starts two days after the beginning of fermentation, which unequivocally demonstrates that those two 18‐residue TB I and TB II peptides with the free carboxy terminus result from enzymatic C‐terminal degradation of the 20‐resido peptides.
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TLDR
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New 19-Residue Peptaibols from Trichoderma Clade Viride
Trichoderma koningiopsis and T. gamsii belong to clade Viride of Trichoderma, the largest and most diverse group of this genus. They produce a wide range of bioactive secondary metabolites, including
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TLDR
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TLDR
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