Sequence variation in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides.

@article{Wyand2005SequenceVI,
  title={Sequence variation in the CYP51 gene of Blumeria graminis associated with resistance to sterol demethylase inhibiting fungicides.},
  author={R. Wyand and J. Brown},
  journal={Fungal genetics and biology : FG \& B},
  year={2005},
  volume={42 8},
  pages={
          726-35
        }
}
  • R. Wyand, J. Brown
  • Published 2005
  • Biology, Medicine
  • Fungal genetics and biology : FG & B
Resistance to sterol 14alpha-demethylase inhibiting fungicides (DMIs) has been correlated with mutations in the CYP51 gene, which encodes the target enzyme eburicol 14alpha-demethylase. To test the hypothesis that variation in the CYP51 gene explains variation for DMI sensitivity in barley and wheat powdery mildew species, this gene was sequenced from isolates of Blumeria graminis f.sp. hordei (Bgh) and f.sp. tritici (Bgt), respectively, which differed in their responses to DMIs in agricultural… Expand
The Y137H mutation in the cytochrome P450 FgCYP51B protein confers reduced sensitivity to tebuconazole in Fusarium graminearum.
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Results indicate that a Tyr137 amino acid mutation in the cytochrome P450 FgCYP51B could lead to resistance to TEC and that Y137 forms part of the tebuconazole-binding pocket. Expand
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References

SHOWING 1-10 OF 48 REFERENCES
The 14alpha-Demethylasse(CYP51A1) Gene is Overexpressed in Venturia inaequalis Strains Resistant to Myclobutanil.
TLDR
The results suggest that overexpression of the target-site CYP51A1 gene is an important mechanism of resistance in some field resistant strains of V. inaequalis, but other mechanisms of resistance also appear to exist. Expand
A mutation in the 14 alpha-demethylase gene of Uncinula necator that correlates with resistance to a sterol biosynthesis inhibitor
TLDR
The molecular basis of resistance of the obligate biotrophic grape powdery mildew fungus Uncinula necator to sterol demethylation-inhibiting fungicides (DMIs) is investigated and a single mutation, leading to the substitution of a phenylalanine residue for a tyrosine residue at position 136, is found. Expand
Genetic control of the resistance of Erysiphe graminis f.sp. hordei to five triazole fungicides
The genetics of resistance to ergosterol demethylation inhibitor (DMI) fungicides of the triazole (conazole) group was examined in a cross between two isolates of the barley powdery mildew fungus,Expand
Tandem Repeat of a Transcriptional Enhancer Upstream of the Sterol 14α-Demethylase Gene (CYP51) inPenicillium digitatum
TLDR
Results indicate that the 126-bp unit acts as a transcriptional enhancer and that a tandem repeat of the unit enhances CYP51 expression, resulting in DMI resistance, a new fungicide resistance mechanism for filamentous fungi. Expand
Genetic control of the response of Erysiphe graminis f.sp. hordei to ethirimol and triadimenol
A study was conducted on the genetic control of the response of Erysiphe graminis f.sp. hordei, the causal agent of barley powdery mildew, to two fungicides: the hydroxypyrimidine ethirimol, and theExpand
Contribution of mutations in the cytochrome P450 14alpha-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans.
TLDR
The cytochrome P450 14alpha-demethylase, encoded by the ERG11 (CYP51) gene, is the primary target for the azole class of antifungals and mutations found in the two N-terminal regions only Y132H was demonstrated to be of importance for azole resistance. Expand
Genetics of responses to morpholine-type fungicides and of avirulences inErysiphe graminis f. sp.hordei
The genetics of the responses of the barley powdery mildew pathogen,Erysiphe graminis f.sp.hordei, to three morpholine-type fungicides were studied. Resistances to a phenylpropylamine fungicide,Expand
Cloning and sequence analysis of the eburicol 14alpha-demethylase gene of the obligate biotrophic grape powdery mildew fungus.
TLDR
In order to obtain molecular data concerning field resistance of Uncinula necator, the causal agent of grape powdery mildew, to sterol demethylation inhibitors, a major group of fungicides, the gene encoding the target of these compounds was cloned and sequenced from this obligately biotrophic phytopathogenic fungus. Expand
Amino Acid Substitutions in the Cytochrome P-450 Lanosterol 14α-Demethylase (CYP51A1) from Azole-Resistant Candida albicans Clinical Isolates Contribute to Resistance to Azole Antifungal Agents
TLDR
It is speculated that specific mutations in the CYP51A1 genes could combine with the effect of multidrug efflux transporters in the clinical isolates and contribute to different patterns and stepwise increases in resistance to azole derivatives. Expand
PCR cloning and detection of point mutations in the eburicol 14a-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a “recalcitrant” fungus
TLDR
A cloning strategy based on PCR has been used to clone the eburicol 14α-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. Expand
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