Sequence-tagged sites (STSs) as standard landmarkers in the rice genome

  title={Sequence-tagged sites (STSs) as standard landmarkers in the rice genome},
  author={Takeshi Inoue and Hongjun Zhong and Akio Miyao and Ikuo Ashikawa and Lisa Monna and Shuichi Fukuoka and N. Miyadera and Yoshiaki Nagamura and Nori Kurata and Tohru Sasaki and Yuzo Minobe},
  journal={Theoretical and Applied Genetics},
Generating sequence-tagged sites (STSs) is a prerequisite to convert a genetic map to a physical map. With the help of sequence information from these STSs one can also isolate specific genes. For these purposes, we have designed PCR primer sets, of 20 bases each, by reference to sequences of restriction fragment length polymorphism (RFLP) landmarkers consisting of rice genomic clones. These markers were evenly distributed over the 12 chromosomes and were shown to be single copy by Southern… CONTINUE READING


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