Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.).

@article{Huettel1999SequencetaggedMS,
  title={Sequence-tagged microsatellite site markers for chickpea (Cicer arietinum L.).},
  author={Bruno Huettel and Peter Winter and Kurt Weising and Wafa Choumane and F. Weigand and G{\"u}nter Kahl},
  journal={Genome},
  year={1999},
  volume={42 2},
  pages={
          210-7
        }
}
Two small-insert genomic libraries of chickpea (Cicer arietinum L.) were screened with a set of microsatellite-specific oligonucleotide probes. A total of 121 positive clones were identified among 13,000 plated colonies. Thirty-nine clones were recognized by (TAA)5, 26 by (GA)8, 18 by (GT)8, 27 by a pool of AT-rich trinucleotide repeats [(CAA)5, (CAT)5, and (GAA)5], and 11 by a pool of GC-rich trinucleotides [(TCC)5, (CAC)5, (CAG)5, and (CGA)5]. Of 53 clones selected for sequencing, 43 carried… CONTINUE READING

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