Sequence specificity of human skin fibroblast collagenase. Evidence for the role of collagen structure in determining the collagenase cleavage site.

@article{Fields1987SequenceSO,
  title={Sequence specificity of human skin fibroblast collagenase. Evidence for the role of collagen structure in determining the collagenase cleavage site.},
  author={Gregg B Fields and Harold E Van Wart and Henning Birkedal-Hansen},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 13},
  pages={
          6221-6
        }
}
The sequence specificity of human skin fibroblast collagenase has been investigated by measuring the rate of hydrolysis of 16 synthetic octapeptides covering the P4 through P4' subsites of the substrate. The choice of peptides was patterned after potential collagenase cleavage sites (those containing either the Gly-Leu-Ala or Gly-Ile-Ala sequences) found in types I, II, and III collagens. The initial rate of hydrolysis of the P1-P1' bond of each peptide has been measured by quantitating the… CONTINUE READING

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