Sequence-specific resonance assignment of soluble nonglobular proteins by 7D APSY-NMR spectroscopy.

@article{Hiller2007SequencespecificRA,
  title={Sequence-specific resonance assignment of soluble nonglobular proteins by 7D APSY-NMR spectroscopy.},
  author={Sebastian Hiller and Christian Wasmer and Gerhard Wider and Kurt W{\"u}thrich},
  journal={Journal of the American Chemical Society},
  year={2007},
  volume={129 35},
  pages={
          10823-8
        }
}
Based on sequence-specific resonance assignments, NMR is the method of choice for obtaining atomic-resolution experimental data on soluble nonglobular proteins. So far, however, NMR assignment of unfolded polypeptides in solution has been a time-consuming task, mainly due to the small chemical shift dispersion, which has limited practical applications of the NMR approach. This paper presents an efficient, fully automated method for sequence-specific backbone and beta-carbon NMR assignment of… CONTINUE READING

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