Sequence-specific isotope effects on the cleavage of DNA by bleomycin.

@article{Kozarich1989SequencespecificIE,
  title={Sequence-specific isotope effects on the cleavage of DNA by bleomycin.},
  author={John W. Kozarich and Leon Worth and Berninger Frank and D F Christner and Dana E. Vanderwall and Joanne Stubbe},
  journal={Science},
  year={1989},
  volume={245 4924},
  pages={1396-9}
}
Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.