Sequence-specific detection of individual DNA polymerase complexes in real time using a nanopore.

@article{Benner2007SequencespecificDO,
  title={Sequence-specific detection of individual DNA polymerase complexes in real time using a nanopore.},
  author={Seico Benner and Roger J A Chen and Noah A. Wilson and Robin Abu-Shumays and Nicholas Hurt and Kate R. Lieberman and David Deamer and William B. Dunbar and Mark Akeson},
  journal={Nature nanotechnology},
  year={2007},
  volume={2 11},
  pages={718-24}
}
Nanoscale pores have potential to be used as biosensors and are an established tool for analysing the structure and composition of single DNA or RNA molecules. Recently, nanopores have been used to measure the binding of enzymes to their DNA substrates. In this technique, a polynucleotide bound to an enzyme is drawn into the nanopore by an applied voltage. The force exerted on the charged backbone of the polynucleotide by the electric field is used to examine the enzyme-polynucleotide… CONTINUE READING