Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1.

@article{Curtis2009SequencespecificDM,
  title={Sequence-specific detection method for reverse transcription, loop-mediated isothermal amplification of HIV-1.},
  author={K. Alexandra Curtis and Donna L. Rudolph and Sherry Michele Owen},
  journal={Journal of medical virology},
  year={2009},
  volume={81 6},
  pages={966-72}
}
HIV diagnosis at the point-of-care or in resource-limited settings poses considerable challenges due to time and cost limitations. Currently, nucleic acid-based tests are the only reliable method for diagnosing recent infections during the window period post-infection and pre-seroconversion, but these tests are only suitable for well-equipped laboratory settings. The reverse transcription loop-mediated isothermal amplification (RT-LAMP) technology exhibits characteristics that are ideal for the… CONTINUE READING

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