Sequence-specific binding of transfer RNA by glyceraldehyde-3-phosphate dehydrogenase.

  title={Sequence-specific binding of transfer RNA by glyceraldehyde-3-phosphate dehydrogenase.},
  author={Rajkumari Singh and Michael R. Green},
  volume={259 5093},
A transfer RNA (tRNA) binding protein present in HeLa cell nuclear extracts was purified and identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Studies with mutant tRNAs indicated that GAPDH recognizes both sequence and structural features in the RNA. GAPDH discriminated between wild-type tRNA and two tRNA mutants that are defective in nuclear export, which suggests that the protein may participate in RNA export. The cofactor nicotinamide adenine dinucleotide… CONTINUE READING