Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution.

@article{Wong2004SequenceSM,
  title={Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution.},
  author={T. S. Wong and K. L. Tee and Berhard Hauer and U. Schwaneberg},
  journal={Nucleic acids research},
  year={2004},
  volume={32 3},
  pages={
          e26
        }
}
Sequence saturation mutagenesis (SeSaM) is a conceptually novel and practically simple method that truly randomizes a target sequence at every single nucleotide position. A SeSaM experiment can be accomplished within 2-3 days and comprises four steps: generating a pool of DNA fragments with random length, 'tailing' the DNA fragments with universal base using terminal transferase at 3'-termini, elongating DNA fragments in a PCR to the full-length genes using a single-stranded template and… Expand
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