Sequence requirements for the editing of apolipoprotein B mRNA.

  title={Sequence requirements for the editing of apolipoprotein B mRNA.},
  author={Rashmi R Shah and Timothy J. Knott and J E Legros and N. Navaratnam and J. Greeve and James M Scott},
  journal={The Journal of biological chemistry},
  volume={266 25},
Apolipoprotein (apo) B48 is produced in the mammalian intestine by a tissue-specific RNA-editing mechanism, which mediates a C to U conversion at position 6666 in apoB mRNA. This generates an inframe translation stop codon (UAA) in place of glutamine (CAA) at position 2153. To establish the sequences required for editing we have used an in vitro conversion assay to monitor the editing of synthetic RNAs by rat intestinal extracts. Transcripts containing 55 nucleotides (positions 6649-6703) or… CONTINUE READING