Sequence requirements for self-splicing of the Tetrahymena thermophila pre-ribosomal RNA.

@article{Price1985SequenceRF,
  title={Sequence requirements for self-splicing of the Tetrahymena thermophila pre-ribosomal RNA.},
  author={James V. Price and G L Kieft and James R. Kent and Eric L. Sievers and Thomas R Cech},
  journal={Nucleic acids research},
  year={1985},
  volume={13 6},
  pages={1871-89}
}
The sequence requirements for splicing of the Tetrahymena pre-rRNA have been examined by altering the rRNA gene to produce versions that contain insertions and deletions within the intervening sequence (IVS). The altered genes were transcribed and the RNA tested for self-splicing in vitro. A number of insertions (8-54 nucleotides) at three locations had no… CONTINUE READING