Sequence requirement and subtype specificity in the high-affinity interaction between human frizzled and dishevelled proteins.

@article{Punchihewa2009SequenceRA,
  title={Sequence requirement and subtype specificity in the high-affinity interaction between human frizzled and dishevelled proteins.},
  author={Chandanamali Punchihewa and Antonio M. Ferreira and Robert Cassell and Patrick Rodrigues and Naoaki Fujii},
  journal={Protein science : a publication of the Protein Society},
  year={2009},
  volume={18 5},
  pages={994-1002}
}
Members of the Wnt family of lipoglycoproteins initiate signaling by binding to Frizzled (Fz) receptors, and the signal is then relayed by Disheveled (Dvl). The Dvl PDZ domain is known to interact directly with a peptide derived from the KTXXXW motif of Fz7, which is conserved in all known Fz subtypes. We found that an extended region spanning the KTXXXW motif on both its N-terminal and C-terminal sides dramatically influences the affinity of peptides derived from Fz7 for Dvl PDZ. An alanine… CONTINUE READING