Sequence-related amplified polymorphism (SRAP) marker as a new method for identification of endophytic fungi from Taxus.

  title={Sequence-related amplified polymorphism (SRAP) marker as a new method for identification of endophytic fungi from Taxus.},
  author={Na Ren and Jiajia Liu and Dongliang Yang and Jianhua Chen and Mingbao Luan and Juan Hong},
  journal={World journal of microbiology & biotechnology},
  volume={28 1},
A total of 20 endophytic fungi stains were classified into four groups using traditional morphological identification method, and were studied for genetic diversity by sequence-related amplified polymorphism (SRAP) technique. Genomic DNA (deoxyribonucleic acid) of these strains was extracted with CTAB method. SRAP analysis was done with 24 pairs of primers. All strains could be uniquely distinguished with 584 bands and 446 polymorphism bands which generated 76.4% of polymorphic ratio… CONTINUE READING

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