Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters

@article{Taris2007SequencePC,
  title={Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters},
  author={Nicolas Taris and Robert P Lang and Mark D. Camara},
  journal={BMC Genomics},
  year={2007},
  volume={9},
  pages={234 - 234}
}
Since it was first described in the mid-1990s, quantitative real time PCR (Q-PCR) has been widely used in many fields of biomedical research and molecular diagnostics. This method is routinely used to validate whole transcriptome analyses such as DNA microarrays, suppressive subtractive hybridization (SSH) or differential display techniques such as cDNA-AFLP (Amplification Fragment Length Polymorphism). Despite efforts to optimize the methodology, misleading results are still possible, even… CONTINUE READING