Sequence patterns produced by incomplete enzymatic digestion or one‐step Edman degradation of peptide mixtures as probes for protein database searches

@article{Jensen1996SequencePP,
  title={Sequence patterns produced by incomplete enzymatic digestion or one‐step Edman degradation of peptide mixtures as probes for protein database searches},
  author={Ole N{\o}rregaard Jensen and Ole Vorm and Matthias Mann},
  journal={ELECTROPHORESIS},
  year={1996},
  volume={17},
  url={https://api.semanticscholar.org/CorpusID:24931903}
}
The technique discussed here is simple, does not require additional measurments, and increases the percentage of protein samples that can be identified by their mass maps alone, and significantly reduces the number of database matches when used in combination with a peptide mass map.
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44 Citations
Background Citations
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Methods Citations
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44 Citations

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The program “PEPTIDE‐MASS”, which generates the theoretical peptide masses of any protein in the SWISS‐PROT database, or of any sequence specified by the user, to better utilize annotated protein databases for the understanding of peptide mass fingerprinting data.

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21 References

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A rapid method for the identification of known proteins separated by two-dimensional gel electrophoresis is described in which molecular masses of peptide fragments are used to search a protein sequence database and each protein was uniquely identified from over 91,000 protein sequences.

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