Sequence organisation in nuclear DNA from Physarum polycephalum: methylation of repetitive sequences.

  title={Sequence organisation in nuclear DNA from Physarum polycephalum: methylation of repetitive sequences.},
  author={Paul A. Whittaker and Anne McLachlan and N. Hardman},
  journal={Nucleic acids research},
  volume={9 4},
Nuclear DNA from the slime mould Physarum polycephalum is digested by the restriction endonuclease HpaII to generate a high molecular weight and a low molecular weight component. These are referred to as the M+ and the M- compartment, respectively. Sequences that are present in the M+ compartment are cleaved by MspI, the restriction enzyme isoschizomer of HpaII, thus showing that the recognition sequences for these enzymes in M+ DNA contain methylated CpG doublets. The distribution of… 
Structural organization of a hypermethylated nuclear DNA component in Physarum polycephalum.
Restriction mapping of cloned Physarum M+ DNA segments, and Southern blot analysis of genomic DNA using subcloned segments of M+DNA as a probe, provide evidence for sequence variation within different copies of the dominant highly repeated element, and possibly the other associated repeats in M- DNA.
Two-dimensional gel analysis of repetitive nuclear DNA sequences in the genome of Physarum polycephalum. Developmental regulation of the ribosomal gene number.
The reduction of several (G + C)-rich repeated sequences in the restricted nuclear DNA in sporulation-competent cells can be explained by a 64% elimination of the extrachromosomal nucleolar ribosomal DNA sequences.
Domains of DNA methylation in eukaryotes : studies on their structure and function
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Sequence complexity of poly(A) RNA fromPhysarum polycephalum
SummaryPoly(A) RNA from S phase, G2 phase and starved macroplasmodia of Physarum contain mRNA sequences which when translated in vitro, yield similar patterns of polypeptides after
Eukaryotic DNA methylation
The introduction of artificially methylated DNA sequences into individual eukaryotic cells by microinjection or transformation may provide the means to elucidate questions in the regulation of gene expression in these organisms.
Molecular Organization of the Physarum Genome
Aside from its intrinsic interest, the molecular characterization of the Physarum genome will have important implications for future studies of the many facets ofphysarum biology.