Sequence organisation in nuclear DNA from Physarum polycephalum: methylation of repetitive sequences.

@article{Whittaker1981SequenceOI,
  title={Sequence organisation in nuclear DNA from Physarum polycephalum: methylation of repetitive sequences.},
  author={Paul A. Whittaker and Anne McLachlan and N. Hardman},
  journal={Nucleic acids research},
  year={1981},
  volume={9 4},
  pages={
          801-14
        }
}
Nuclear DNA from the slime mould Physarum polycephalum is digested by the restriction endonuclease HpaII to generate a high molecular weight and a low molecular weight component. These are referred to as the M+ and the M- compartment, respectively. Sequences that are present in the M+ compartment are cleaved by MspI, the restriction enzyme isoschizomer of HpaII, thus showing that the recognition sequences for these enzymes in M+ DNA contain methylated CpG doublets. The distribution of… 
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