Sequence of late molecular events in the activation of rhodopsin.

  title={Sequence of late molecular events in the activation of rhodopsin.},
  author={Bernhard Knierim and Klaus Peter Hofmann and Oliver P Ernst and Wayne L. Hubbell},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={104 51},
Activation of the G protein-coupled receptor rhodopsin involves both the motion of transmembrane helix 6 (TM6) and proton exchange events. To study how these activation steps relate to each other, spin-labeled rhodopsin in solutions of dodecyl maltoside was used so that time-resolved TM6 motion and proton exchange could each be monitored as a function of pH and temperature after an activating light flash. The results reveal that the motion of TM6 is not synchronized with deprotonation of the… CONTINUE READING


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