Sequence of an osmotically inducible lipoprotein gene.

  title={Sequence of an osmotically inducible lipoprotein gene.},
  author={Jae U Jung and Claude Gutierrez and Merna Villarejo},
  journal={Journal of bacteriology},
  volume={171 1},
The osmB gene of Escherichia coli, whose expression is induced by elevated osmolarity, was cloned and physically mapped to a 0.65-kilobase-pair NsiI-HincII DNA fragment at 28 min on E. coli chromosome. The OsmB protein was identified in minicells expressing the cloned gene. The nucleotide sequence of a 652-base-pair chromosomal DNA fragment containing the osmB gene was determined. The open reading frame encodes a 72-residue polypeptide with an Mr of 6,949. This reading frame was confirmed by… CONTINUE READING


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