Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate.

@article{Malissard1993SequenceOA,
  title={Sequence of a gene encoding a (poly ManA) alginate lyase active on Pseudomonas aeruginosa alginate.},
  author={M Malissard and Colette Duez and M Guinand and M J. Vacheron and Georges Michel and Nicole Marty and Bernard Joris and Iris Thamm and J. M. Ghuysen},
  journal={FEMS microbiology letters},
  year={1993},
  volume={110 1},
  pages={101-6}
}
The recombinant plasmid pAL-A3 bears a (poly ManA) alginate lyase-encoding gene that originates from the marine bacterium ATCC 433367 (Brown et al., Appl. Environ. Microbiol. (1991) 57, 1870-1872). The alginate lyase produced by Escherichia coli TC4 harbouring pAL-A3 was purified to protein homogeneity and the corresponding gene sequenced, giving access to the first known primary structure of an alginate lyase. The 265-amino acid residue alginate lyase showed lytic activity on a Pseudomonas… CONTINUE READING