Sequence of a gene (lap) encoding a 95.3-kDa aminopeptidase from Lactococcus lactis ssp. cremoris Wg2.


A gene (lap) coding for a Lactococcus lactis ssp. cremoris Wg2 aminopeptidase was cloned from genomic libraries of size-fractionated lactococcal DNA. The 5' end of the lap gene was isolated by using a polymerase chain reaction hybridization probe of 77 nucleotides (nt) synthesized from two degenerate primers derived from the N-terminal amino acid (aa… (More)


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