Sequence of a complete chicken delta-crystallin cDNA.

  title={Sequence of a complete chicken delta-crystallin cDNA.},
  author={John M. Nickerson and Joram Piatigorsky},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={81 9},
A full-length chicken delta-crystallin cDNA (p delta Cr17) was cloned and subjected to sequence analysis. The cDNA was shown to be full-length by both primer extension and S1 and mung bean nuclease experiments. Thus, the complete amino acid sequence of delta-crystallin is now available. The delta-crystallin polypeptide has a molecular mass of 48,542 daltons, as expected from its behavior on NaDodSO4/polyacrylamide gels. delta-Crystallin has one tryptophan, no cysteines, a prevalence of leucines… CONTINUE READING