Sequence-independent, single-primer amplification (SISPA) of complex DNA populations.

  title={Sequence-independent, single-primer amplification (SISPA) of complex DNA populations.},
  author={Gregory R. Reyes and Jung-suh P. Kim},
  journal={Molecular and cellular probes},
  volume={5 6},
Sequence-Independent, Single-Primer Amplification (SISPA) is a primer initiated technique that requires target sequence modification to achieve the non-selective logarithmic amplification of heterogeneous DNA populations. The method contrasts with the polymerase chain reaction (PCR), and its modified approaches, that have as their objective the amplification of unique or homologous sequences. SISPA requires the directional ligation of an asymmetric linker/primer oligonucleotide onto the target… CONTINUE READING


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