Sequence elements outside the catalytic core of natural hairpin ribozymes modulate the reactions differentially.

@article{Bajaj2011SequenceEO,
  title={Sequence elements outside the catalytic core of natural hairpin ribozymes modulate the reactions differentially.},
  author={Preeti Bajaj and Gerhard Steger and Christian Hammann},
  journal={Biological chemistry},
  year={2011},
  volume={392 7},
  pages={593-600}
}
Abstract Hairpin ribozymes occur naturally only in the satellite RNAs of tobacco ringspot virus (TRsV), chicory yellow mottle virus (CYMoV) and arabis mosaic virus (ArMV). The catalytic centre of the predominantly studied sTRsV hairpin ribozyme, and of sArMV is organised around a four-way helical junction. We show here that sCYMoV features a five-way helical junction instead. Mutational analysis indicates that the fifth stem does not influence kinetic parameters of the sCYMoV hairpin ribozyme… CONTINUE READING