Sequence diversity of internal transcribed spacer-1 (ITS-1) region of Eimeria infecting chicken and its relevance in species identification from Indian field samples

@article{Bhaskaran2009SequenceDO,
  title={Sequence diversity of internal transcribed spacer-1 (ITS-1) region of Eimeria infecting chicken and its relevance in species identification from Indian field samples},
  author={Mohana Subramanian Bhaskaran and Lakshmipriya Venkatesan and Ramesh Aadimoolam and Harikrishnan Tirunelveli Jayagopal and Rajan Sriraman},
  journal={Parasitology Research},
  year={2009},
  volume={106},
  pages={513-521}
}
Conventional method of species identification in Eimeria employs phenotypic characters of the oocysts and the site of infection in the chicken intestine, which are subjective analyses. PCR-based identification of Eimeria spp. is known to be specific and sensitive. We used internal transcribed spacer 1 (ITS-1)-based nested PCR to follow the distribution of Eimeria spp. in the field, which may be of significant value in the management of coccidiosis in chickens. In the present study, intestinal… CONTINUE READING