Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA.

@article{Hattori2001SequenceCO,
  title={Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA.},
  author={Yuuki Hattori and Kazuya Iwata and Kouhei Suzuki and Misugi Uraji and Niji Ohta and Akino Katoh and Kakoto Yoshida},
  journal={Genes & genetic systems},
  year={2001},
  volume={76 2},
  pages={
          121-30
        }
}
We isolated a crown gall tumor-inducing nopaline type Ti plasmid from Agrobacterium tumefaciens on a Sakura Japanese cherry tree, and designated it as pTi-SAKURA. By primer walking sequencing with long PCR and a newly developed PCR subcloning technique for long insert DNA, we completed DNA sequencing of the most important functional unit, the virulence (vir) region of pTi-SAKURA, which is indispensable for T-DNA transfer into the plant's chromosomes. By homology searches with the vir genes of… CONTINUE READING

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