Sequence capture using PCR-generated probes: a cost-effective method of targeted high-throughput sequencing for nonmodel organisms.

@article{Pealba2014SequenceCU,
  title={Sequence capture using PCR-generated probes: a cost-effective method of targeted high-throughput sequencing for nonmodel organisms.},
  author={Joshua V. Pe{\~n}alba and Lydia L. Smith and Maria A. Tonione and Chodon Sass and Sarah M. Hykin and Phillip L Skipwith and Jimmy A Mcguire and Rauri C. K. Bowie and Craig Moritz},
  journal={Molecular ecology resources},
  year={2014},
  volume={14 5},
  pages={1000-10}
}
Recent advances in high-throughput sequencing library preparation and subgenomic enrichment methods have opened new avenues for population genetics and phylogenetics of nonmodel organisms. To multiplex large numbers of indexed samples while sequencing predominantly orthologous, targeted regions of the genome, we propose modifications to an existing, in-solution capture that utilizes PCR products as target probes to enrich library pools for the genomic subset of interest. The sequence capture… CONTINUE READING