Sequence-based classification scheme for the genus Legionella targeting the mip gene.

  title={Sequence-based classification scheme for the genus Legionella targeting the mip gene.},
  author={Rodney Mark Ratcliff and J. A. Lanser and Paul A. Manning and Michael W. Heuzenroeder},
  journal={Journal of clinical microbiology},
  volume={36 6},
The identification and speciation of strains of Legionella is often difficult, and even the more successful chromatographic classification techniques have struggled to discriminate newly described species. A sequence-based genotypic classification scheme is reported, targeting approximately 700 nucleotide bases of the mip gene and utilizing gene amplification and direct amplicon sequencing. With the exception of Legionella geestiana, for which an amplicon was not produced, the scheme clearly… CONTINUE READING

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