Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms.

Abstract

The APH gene of a butirosin-producing Bacillus circulans was cloned and shown to be expressed in Escherichia coli and Streptomyces lividans. The gene was sequenced and a possible developmentally regulated promoter identified. When the deduced protein sequence was compared with those from transposon Tn5, transposon Tn903, Streptomyces fradiae, Staphylococcus aureus and Streptococcus faecalis, significant homology was found, indicating that the genes may have a common origin.

Cite this paper

@article{Herbert1986SequenceAI, title={Sequence and interspecies transfer of an aminoglycoside phosphotransferase gene (APH) of Bacillus circulans. Self-defence mechanism in antibiotic-producing organisms.}, author={Christopher J. Herbert and Mohammed . Sarwar and Sarbjit S. Ner and I. G. Giles and Muhammad Mohsin Akhtar}, journal={The Biochemical journal}, year={1986}, volume={233 2}, pages={383-93} }