Sequence and expression of rat ICAM-1.

  title={Sequence and expression of rat ICAM-1.},
  author={Yusuke Kita and Tohru Takashi and Yutaka Iigo and Takashi Tamatani and Masayuki Miyasaka and Tadashi Horiuchi},
  journal={Biochimica et biophysica acta},
  volume={1131 1},
We have isolated cDNA clones-coding for rat intercellular adhesion molecule-1 (RICAM-1) from a cDNA library constructed from rat Ax cells stimulated with IL-1 beta using the mouse ICAM-1 cDNA as a hybridization probe. The RICAM-1 sequence shows 79.1% homology with mouse ICAM-1 and 55.6% homology with human ICAM-1 at the nucleic acid level. In order to examine the expression of RICAM-1 on Chinese hamster ovary (CHO) cells, we constructed the vector, pSV-RICAM1-neo, containing the SV40 promoter… CONTINUE READING

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