Sequence and diversity of DRB genes of Aotus nancymaae, a primate model for human malaria parasites

  title={Sequence and diversity of DRB genes of Aotus nancymaae, a primate model for human malaria parasites},
  author={J. Javier Nino-Vasquez and Denise Vogel and Ra{\'u}l Rodr{\'i}guez and Alberto Moreno and Manuel Elkin Patarroyo and Gerd Pluschke and Claudia A. Daubenberger},
Abstract The New World primate Aotus nancymaae is susceptible to infection with the human malaria parasite Plasmodium falciparum and Plasmodium vivax and has therefore been recommended by the World Health Organization as a model for evaluation of malaria vaccine candidates. We present here a first step in the molecular characterization of the major histocompatibility complex (MHC) class II DRB genes of Aotus nancymaae (owl monkey or night monkey) by nucleotide sequence analysis of the… 

Sequence and expression of MHC-DPB1 molecules of the New World monkey Aotus nancymaae, a primate model for Plasmodium falciparum

The nucleotide sequences of exon 2 and 3 of MHC-DPB1 genes are presented and no evidence could be found for the conservation of allelic lineages pre-dating the divergence of Old and New World monkeys.

Sequence and diversity of MHC DQA and DQB genes of the owl monkey Aotus nancymaae

The results fully support the use of Aotus monkeys as an animal model for evaluation of future subunit vaccine candidates, and essential amino acid residues contributing to MHC DQ peptide binding pockets number 1 and 4 are conserved or semi-conserved between HLA-DQ and Aona-D Q molecules, indicating a capacity to bind similar peptide repertoires.

Characterising a Microsatellite for DRB Typing in Aotus vociferans and Aotus nancymaae (Platyrrhini)

Non-human primates belonging to the Aotus genus have been shown to be excellent experimental models for evaluating drugs and vaccine candidates against malaria and other human diseases. The immune

Sequence variability analysis on major histocompatibilitycomplex class II DRB alleles in three felines

Results displayed a more close relationship between leopard and tiger; however, clouded leopard has a comparatively distant relationship form the other two; and MEGA neighbor joining and PAUP maximum parsimony methods were used to reconstruct phylogenetic trees among species.

Sequence variation and gene duplication at the MHC DRB loci of the spotted seal Phoca largha.

High level of spotted seal MHC-DRB polymorphism revealed in the present study has not been reported for the Phocidae and could be a consequence of the small spotted seal population adapting to the Bohai Sea, which probably has a relatively high level of pathogens.

Identifying and structurally characterizing CD1b in Aotus nancymaae owl monkeys

This study reports the molecular characterization and tissue expression of the non-human Aotus nancymaae primate CD1b isoform in the search for an experimental animal model to be used in evaluating

Structural and functional characterisation of the Toll like receptor 9 of Aotus nancymaae, a non-human primate model for malaria vaccine development

The high level of sequence conservation of Aona-TLR-9 reinforces the suitability of A. nancymaae as animal model for malaria subunit vaccine development and selects between different formulations before conducting expensive human clinical trials.

Sequence variation and gene duplication at MHC DQB loci of baiji (Lipotes vexillifer), a Chinese river dolphin.

The high level of baijin MHC polymorphism revealed in the present study has not been reported in other cetaceans and could be a consequence of the small baiji population adapting to freshwater with a relatively high levelof pathogens.

Major histocompatibility complex class II DR and DQ evolution and variation in wild capuchin monkey species (Cebinae)

High-throughput sequencing is used to characterize polymorphism in four Class II DR and DQ exons for the first time in capuchin species, and phylogenetic analyses reveal that platyrrhine DQA sequences form a monophyletic group to the exclusion of all Catarrhini sequences examined.



Sequence and diversity of T-cell receptor β-chain V and J genes of the owl monkey Aotus nancymaae

The results confirm that the TCR repertoire in primates is remarkably stable and support the concept of using Aotus monkeys as an infection model for the evaluation of future subunit vaccine candidates.

The common marmoset: A new world primate species with limited Mhc class II variability

The results demonstrate that the common marmoset has, like other primates, apparently functional Mhc-DR and -DQ regions, but the MhC-DP region has been inactivated, and an unexpectedly high number of allelic combinations are observed at the haplotypic level, suggesting that Caja-DRB alleles are exchanged frequently between chromosomes by recombination, promoting an optimal distribution of limited M hc polymorphisms among individuals of a given population.

Polymorphism of Mhc-DRB alleles in Cercopithecus aethiops (green monkey): generation and functionality.

The eleven green monkey DRB alleles meet the requirements for functionality as antigen-presenting molecules (perhaps, excluding DRB1*0701), since they have been isolated from cDNA and do not present deletions, insertions or stop codons.

Evolutionary conservation of major histocompatibility complex- DR/peptide/T cell interactions in primates

It is demonstrated that some polymorphic amino acid residues (motifs) within the antigen-binding site of MHC class II molecules that are crucial for peptide binding and recognition by the T cell receptor have been conserved for over 30 million years.

Allelic diversity is generated by intraexon sequence exchange at the DRB1 locus of primates.

It is revealed that sequences encoding the beta-pleated sheet and those encoding the alpha-helix of the second domain have different evolutionary histories and may have been generated in part by combining different variants of the two structural domains.

The origin of the primate Mhc-DRB genes and allelic lineages as deduced from the study of prosimians.

The partially sequenced genes of the DRB family revealed that the primate DRB region is structurally and functionally unstable and several of the prosimian DRB genes and pseudogenes have recently been duplicated.

Phylogenetic history of hominoid DRB loci and alleles inferred from intron sequences

The phylogenetic trees for these sequences indicate that most of the DRB 1 allelic lineages predate the separation of the hominoid species studied, consistent with previous analysis of the coding sequences of these lineages.

Ancestral major histocompatibility complex DRB genes beget conserved patterns of localized polymorphisms.

  • L. GaurG. Nepom
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1996
This work identified nucleotide elements within macaque and baboon DRB6-like sequences with deletions corresponding to specific exon 2 hypervariable regions, which encode a discrete alpha helical segment of the MHC antigen combining site.

Evolution of Major Histocompatibility Complex Polymorphisms and T‐Cell Receptor Diversity in Primates

The self-sustaining nonhuman primate colonies at the institute have been pedigreed based on Mhc genetics and their outbred character has been carefully maintained for more than 20 years.

Origin of major histocompatibility complex polymorphism: the trans-species hypothesis.