Sequence analysis of three novel DRw14-DRB1 alleles

  title={Sequence analysis of three novel DRw14-DRB1 alleles},
  author={R. Dong and A. Kimura and T. Sasazuki},
The functional HLA class II molecules comprising DR, DQ, and DP provide human populations with a diversity of immune responses to natural antigens and control susceptibility and resistance to some diseases. One of the characteristic features of HLA class II molecules is their extensive polymorphism in the first extracellular domain encoded by the second exon. Molecular and genetic studies on the polymorphism have recently been examined at the DNA level, using polymerase chain reaction (PCR… Expand
1 Citations
Nomenclature for factors of theHLA system, 1991
The WHO Nomenclature Committee for factors of the HLA system met in Hakone after the 1 lth International Histocompatibility Workshop and Conference during November 1991 to consider additions andExpand


DRB1*LY10 — a newDRB1 allele and its haplotypic association
The sequence of a new DRB1 allele, DRBI*LYIO, and its haplotypic association is reported, which was made during an HLA analysis of a patient with lyme arthritis and revealed a new hybridization pattern which suggested that the individual carried theDRB1*0402 (DR4DwlO) allele on one chromosome and a new allele on the other chromosome. Expand
Allelic diversity is generated by intraexon sequence exchange at the DRB1 locus of primates.
It is revealed that sequences encoding the beta-pleated sheet and those encoding the alpha-helix of the second domain have different evolutionary histories and may have been generated in part by combining different variants of the two structural domains. Expand
First domain sequence of the HLA-DRB1 chain from two HLA-DRw14 homozygous typing cell lines: TEM (Dw9) and AMALA (Dw16).
  • J. Gorski
  • Biology, Medicine
  • Human immunology
  • 1989
First domain sequences of the B1 locus reveal a cluster of polymorphisms in the third hyperpolymorphic region indicative of gene conversion-like mechanisms in the DRw14 specificity. Expand
Sequence analysis and HLA-DR genotyping of a novel HLA-DRw14 allele
Analysis of a Japanese population by oligonucleotide genotyping revealed that one Japanese HLA-DRw14 allele had a DRB1 genotype different from that of the known HLA-DRw14-related alleles, DRB1*1401Expand
Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes
The polymerase chain reaction (PCR) procedure is used to enzymatically amplify a specific segment of the β-globin or HLA-DQα gene in human genomic DNA before hybridization with ASOs, enabling the analysis of allelic variation with as little as 1 ng of genomic DNA and the use of a simple ‘dot blot’ for probe hybridization. Expand
DRw8 microvariation: a new DRB1 allele identified in association with DQw7 in American blacks.
In the American black population, DRw8 is commonly found in association with DQw7 and an undefined HLA-D specificity. Polymerase chain reaction amplification was used to obtain DRB1 cDNA clones fromExpand
Single amino acid changes in DR and antigen define residues critical for peptide-MHC binding and T cell recognition.
The observed pattern of complementation led to a model that predicts that the Ag assumes an extended conformation, with a turn, in the binding groove, such that the following residues are in close proximity: DR 86-HA 309, DR 71-HA 312, DR 30-HA 314, and 315. Expand
Sequence analysis and oligonucleotide genotyping of HLA-DR"JX6", a DR"blank" haplotype found in the Japanese population.
One of the HLA-DR"blank" haplotypes found in the Japanese population is analyzed using serologic studies, sequence determination, and genotyping with sequence-specific oligonucleotide (SSO) probes and found to show a hybridization profile that was consistent with the results of serologic Studies on the DR"JX6" haplotype. Expand
HLA class II nucleotide sequences, 1991.
  • S. Marsh, J. Bodmer
  • Biology, Medicine
  • European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics
  • 1991
The HLA class II sequences included in this compilation are taken from publications listed in the accompanying paper, Nomenclature for factors of the HLA system, 1990 (Bodmer et al., 1991), andExpand
HLA class II nucleotide sequences, 1991
The HLA class II sequences included in this compilation are taken from publications listed in the accompanying paper, Nomenclature for factors of the HLA system, 1990 (Bodmer et al. 1991), and necessary amendments to published sequences have been incorporated into this alignment. Expand