Sequence analysis of three family B DNA polymerases from the thermoacidophilic crenarchaeon Sulfurisphaera ohwakuensis.

@article{Iwai2000SequenceAO,
  title={Sequence analysis of three family B DNA polymerases from the thermoacidophilic crenarchaeon Sulfurisphaera ohwakuensis.},
  author={Toshie Iwai and Nobuyuki Kurosawa and Yuko H. Itoh and Natsuki Kimura and Tadao Horiuchi},
  journal={DNA research : an international journal for rapid publication of reports on genes and genomes},
  year={2000},
  volume={7 4},
  pages={243-51}
}
Three family B DNA polymerase genes, designated B1, B2, and B3, were cloned from the thermoacidophilic crenarchaeon Sulfurisphaera ohwakuensis, and sequenced. Deduced amino acid sequences of B1 and B3 DNA polymerases have all exonuclease and polymerase motifs which include critical residues for catalytic activities. Furthermore, a YxGG/A motif, which is located between 3'-5' exonuclease and polymerization domains of family B DNA polymerases, was also found in each of the B1 and B3 sequences… CONTINUE READING
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